Non-destructive raman spectroscopy for detecting AM fungal colonization in crops
A non-invasive Raman spectroscopy method to detect arbuscular mycorrhizal (AM) fungal root colonization in crops, offering growers immediate in-field feedback without expert input. Validated with >80% accuracy, this technology supports efficient AM fungal management on farms.
Overview
The proposed technology is a non-destructive Raman spectroscopic solution designed to detect arbuscular mycorrhizal (AM) fungal colonization in crop roots by analyzing leaf spectra. This innovative method offers growers a practical, automated approach to monitor AM fungal interactions in their fields, providing immediate feedback without the need for costly laboratory assays. By detecting pigment-level changes associated with AM fungal symbiosis, the technology aids in in-season decision-making and enhances confidence in AM fungal interventions across diverse soils and large grower networks.
Technical specifications
The technology utilizes Raman spectroscopy to non-invasively image plant leaves, inferring the AM fungal colonization status of roots. Machine learning models, trained with data from multiple plant species grown with and without AM fungi, achieve over 80% accuracy using partial least squares-discriminant analysis. The system captures physiological changes in plants resulting from AM fungal interactions, providing a reliable measure of root colonization.
Key features:
- Non-destructive, in-field sensing approach
- Over 80% accuracy in detecting AM fungal colonization
- Applicable across multiple plant species and soil types
- Supports real-time monitoring and decision-making
Technology readiness level
The technology is currently at TRL 4, having undergone validation with five plant species under controlled conditions. Future development will focus on expanding crop species validation and conducting field trials to enhance robustness and applicability. This will involve further testing across different AM fungal communities and environmental conditions to ensure comprehensive assessment capabilities.
About The Ohio State University
The Ohio State University is a comprehensive public land‑grant research university in Columbus, serving one of the nation’s largest student populations and a broad research enterprise. Industry partners engage through an integrated academic medical center for clinical translation, a campus‑adjacent innovation district for co‑located projects, and a statewide extension network that pilots solutions across Ohio. Corporate engagement provides a single front door for sponsored research, talent pipelines, and streamlined agreements. Research is supported by competitive federal funding from agencies such as NIH, NSF, DOE, USDA, DoD, and NASA. A dedicated technology transfer office and venture support help protect IP, license technologies, and launch startups.
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