One-pot pathogen detection preparation using ionic liquids and labile surfactants
Innovative one-pot preparation method using ionic liquids and labile surfactants for pathogen detection in food matrices. This approach simplifies sample preparation by enabling single-step lysis and isolation of molecular markers, enhancing efficiency and scalability for testing.
Overview
The one-pot preparation method utilizes a novel combination of bacteriolytic ionic liquids and labile surfactants to efficiently isolate pathogen molecular markers from complex food matrices. This innovative solution enhances the scalability and efficiency of pathogen detection by simplifying the sample preparation process. The use of chemical and incubation methods provides a stable, single-step lysis and isolation of microbial samples without the need for physical disruption, making it highly suitable for industrial applications.
Technical specifications
- Key components: Utilizes [C2mim]OAc ionic liquid for direct lysis of Gram-positive and Gram-negative bacteria, combined with DDM (n-dodecyl-beta-Maltoside), a pH and temperature labile surfactant.
- Optimization: Includes optimizing ionic liquid and surfactant composition, incubation conditions, and additional additives such as chaotropic salts.
- Application methods: Procedures optimized for addition of heat/time, full inactivation, and potentially filter binding for inhibitor depletion or analyte enrichment.
- Detection techniques: Compatible with antigen detection, PCR, and mass spectrometry methods.
Technology readiness level
Currently, this technology is at TRL 3, indicating that it has been validated in a laboratory environment. Further validation and optimization are planned, including testing with various microbial-foodstuff simulations and different detection methods to ensure robustness and reliability.
About University of Notre Dame
The University of Notre Dame is a private, comprehensive research university with global reach and a residential campus in Notre Dame, Indiana. Industry engages on campus and nearby through a research and technology park, an incubator, and shared core labs for prototyping, characterization, and scale-up testing. Large testbeds and pilot facilities let partners validate systems under realistic conditions, while corporate engagement teams streamline sponsored research and talent pipelines. Faculty win competitive federal funding from agencies such as the National Science Foundation, National Institutes of Health, the Department of Energy, and the Department of Defense. Technology transfer supports IP, licensing, and startup formation via industry-friendly agreements.
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