Optimized solvent-based extraction for mycotoxin detection in grains
A novel mycotoxin detection method utilizing optimized solvent polarity for efficient extraction and HPLC analysis of grain contaminants. This approach enhances separation and identification of diverse mycotoxins, ensuring food safety.
Overview
This innovative method enhances mycotoxin detection in grains by optimizing solvent polarity for extraction, coupled with high-performance liquid chromatography (HPLC) analysis. By tailoring the solvent mixture based on polarity indices, the method improves the extraction efficiency of a wide range of mycotoxins, including aflatoxins and ochratoxins, ensuring comprehensive food safety monitoring.
Technical specifications
- Extraction solvents: Methanol, acetonitrile, and water are used based on their polarity indices to optimize mycotoxin extraction.
- HPLC process: Mobile phase mixtures are optimized for different mycotoxins using retention factors to improve separation and identification.
- Mycotoxin diversity: Capable of detecting various mycotoxins with differing solubility profiles, enhancing detection accuracy.
- Calibration curve: Standard mycotoxin solutions are used to plot a calibration curve for precise quantification.
Technology readiness level
This technology is at a TRL 6, indicating that it has been validated in a relevant environment and is ready for further development towards market readiness.
About Purdue University, Northwest
Purdue University Northwest is a comprehensive metropolitan public university with campuses in Hammond and Westville serving the Chicago–Northwest Indiana industrial corridor. Industry engagement is anchored by on‑campus applied research assets like the Center for Innovation through Visualization and Simulation, offering advanced modeling and AR/VR visualization for plant‑scale problem‑solving. CMEC provides pilot manufacturing space, training, and support for product and prototype development near campus. Research is backed by competitive federal and state funding, including the National Science Foundation and the U.S. Department of Energy, plus industry partnerships. IP, licensing, and startups are supported through the Purdue Research Foundation’s Office of Technology Commercialization.
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