Portable fluorometric sensor for on-site detection of Salmonella typhimurium
This portable sensor uses a nanoparticle-catalyzed fluorogenic assay (NCFA) for point-of-use detection of Salmonella typhimurium. It offers high sensitivity and rapid results without needing sophisticated instruments, ideal for complex food matrices like chocolate.
Overview
The portable fluorometric sensor is designed for the point-of-use detection of the bacterial pathogen Salmonella typhimurium. Utilizing a cutting-edge nanoparticle-catalyzed fluorogenic assay (NCFA), this sensor provides high sensitivity and rapid detection capabilities. The envisioned product is a user-friendly kit, including a test strip, reagents, and a compact fluorescence reader, making it ideal for complex food matrices such as chocolate.
Technical specifications
- NCFA Technology: Utilizes nanoparticle-catalyzed signal amplification for ultra-high sensitivity.
- Detection Workflow: Involves dipping a test strip into a sample and reagents followed by reading with a palm-sized fluorescence reader.
- Components: Includes a pair of oligo probes (P1 & P2) and a DNA aptamer specific to S. typhimurium.
- Materials: Glass fiber is used for test strips, with options for alternative materials.
- Applications: Suitable for detecting S. typhimurium in various food products, particularly chocolate.
Technology readiness level
This technology is currently at TRL 4, with successful lab demonstrations and ongoing optimization for point-of-use applications. Further validation in real-world conditions is planned to enhance its market readiness.
About University of Cincinnati
The University of Cincinnati is a comprehensive public research university with an applied, urban-serving character and a significant clinical enterprise. Industry engages through one of the nation's largest cooperative education programs, placing students year-round with corporate R&D and operations teams and creating an on-ramp to sponsored research. An innovation district near campus hosts co-located corporate labs, startup space, and shared prototyping facilities, while the university's integration with a major hospital system enables clinical studies and translation. Research is supported by competitive federal funding from agencies such as NIH and NSF, along with state and industry partnerships. A dedicated technology transfer office manages IP, licensing, corporate agreements, and startup formation, providing flexible models for collaboration.
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