Powdery mildew-resistant wine grapevines with RUN1 and REN1 genetic markers

Overview

This solution offers advanced grapevine lines developed through a decade-long pseudo-backcrossing breeding program that introduces the RUN1 gene and REN1 resistance locus into premium Vitis vinifera wine cultivars. The technology addresses one of the most persistent challenges in viticulture: infection by Erysiphe necator, the fungus responsible for powdery mildew. By combining genetic resistance with commercially desirable oenological and organoleptic characteristics, these grapevine selections aim to reduce fungicide dependency while maintaining wine quality standards expected by the industry.

The opportunity is particularly relevant for wine producers seeking sustainable disease management, reduced input costs, and varieties that meet both agronomic and market demands.

Technical specifications

Key features:

• Genetic resistance: Incorporation of RUN1 gene and REN1 locus conferring resistance to Erysiphe necator infection
• Breeding methodology: Pseudo-backcrossing approach refined over 10 years of laboratory research
• Quality focus: Selection for favorable physiological and organoleptic fruit characteristics suitable for wine production
• Verification methods: PCR analysis for RUN1 gene presence and SSR (Simple Sequence Repeat) analysis for REN1 locus confirmation
• Phenotypic validation: Field-based resistance screening combined with fruit, must, and wine quality analysis

Development approach:

• Directed pollinations between agronomically desirable plants and genetically resistant parents
• Progeny screening through genetic marker verification
• Agricultural field trials to confirm phenotypic resistance
• Comprehensive wine production and quality evaluation from resistant lines

Technology readiness level

The program is at an advanced stage of development, with successful pseudo-backcrossing already achieved and current selections demonstrating genetic presence of both RUN1 and REN1 markers. Field-level resistance to Erysiphe necator has been observed in advanced selections. Ongoing work focuses on optimizing commercial characteristics through continued directed breeding, with genetic verification, phenotypic field studies, and wine quality assessments forming the validation pipeline. The technology is positioned between laboratory validation and pre-commercial field trials, with readiness advancing as progeny with combined resistance and desirable commercial traits are identified and characterized.