Precision gene editing: agrobacterium and protoplast solutions

The Protoplast Advantage and the DNA-Free Future As global regulations evolve, protoplast regeneration is becoming the gold standard for rapid, non-transgenic gene editing. By isolating individual cells and removing the cell wall, we enable direct access to the genome for unparalleled precision. We can transfect these protoplasts with either DNA vectors or Ribonucleoprotein (RNP) complexes, facilitating a DNA-free approach that avoids the insertion of foreign genetic material. This streamlined path results in a single edited protoplast being regrown into an entire non-transgenic plant, which significantly eases the journey toward regulatory approval and commercialization. Mastery of the Regeneration Challenge Protoplast regeneration remains one of the most challenging aspects of plant biotechnology, but Solis has onboarded and developed specialized protocols across many crops. We understand the key parameters that must be tested with each new variety to ensure success. Our comprehensive onboarding process for new crops and varieties follows a rigorous four-step framework: digestion for cell wall removal, transfection for reagent delivery, editing validation, and final regeneration. This methodical approach ensures we reach event delivery with the highest efficiency possible. Ready to accelerate your trait development? Whether you need the established reliability of Agrobacterium or the cutting-edge regulatory speed of protoplast regeneration, Solis provides the end-to-end support to take your project from sequence to seed.