Preparation-free autonomous environmental DNA ddpcr biosensor
NS2 has developed an autonomous biosensor for real-time eDNA detection using ddPCR. It integrates sample collection, preparation, and quantification into a single unit ideal for remote environments, enabling transformative applications in water quality monitoring.
Overview
Nucleic Sensing Systems (NS2) has developed an innovative biosensor platform that revolutionizes the detection of environmental DNA (eDNA) using droplet digital PCR (ddPCR). This autonomous, field-deployable solution is specifically designed for remote and challenging environments such as aquaculture systems, wastewater treatment plants, and natural water bodies. By automating the entire process from sample collection to target quantification, NS2's biosensor eliminates the need for laboratory infrastructure, cold-chain logistics, and skilled technicians, making it a transformative solution for decentralized and high-impact environmental monitoring.
Technical specifications
Key features:
- Fully autonomous operation integrating sample collection, preparation, droplet generation, and quantification.
- Automated filtration, lysis, nucleic acid purification, and mixing protocols optimized for low-concentration targets.
- Ruggedized design suitable for continuous deployment in harsh environments.
- Eliminates the need for sample preparation by analyzing "free eDNA," enhancing efficiency and reducing processing time.
- Future enhancements include a concentration module to significantly reduce sample volumes and improve ddPCR compatibility.
Technology readiness level
This biosensor platform is currently at TRL 4. It has been validated in lab environments, and further phases are planned to adapt and optimize the system for advanced sample preparation and field deployment, engaging end-users for continuous feedback.
About Nucleic Sensing Systems (NS2)
NS2 is a startup founded by scientists and engineers from the University of Minnesota. Since around 2019, it has been developing cutting-edge biosensors for water quality and biosecurity. It’s a participant in water-tech accelerators (e.g., Imagine H2O) and has received recognition for its innovation in environmental monitoring.
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