Probiotic stability enhancement with dtpa hydrophilin technology

Overview

HydroBiome's innovative DtpA hydrophilin technology aims to revolutionize the probiotic industry by significantly enhancing the stability and efficacy of probiotic strains. This technology modifies probiotics to withstand desiccation, heat, and oxidative stress, ensuring higher colony-forming unit (CFU) counts throughout the supply chain. By improving the survivability of probiotics, DtpA provides a more reliable and effective product for consumers, extending shelf life and maintaining potency during manufacturing, shipping, and storage.

Technical specifications

Key features:

• DtpA hydrophilin technology: Enhances desiccation and heat tolerance in probiotics
• Genetic modification: DtpA expressed in E.coli Nissle (EcN) demonstrates increased desiccation tolerance and recovery in vivo
• Versatile application: Planned integration with Lactobacillus and Bifidobacterium strains

Applications:

• Gummy probiotics: Compatibility with gelatin and pectin gummy manufacturing processes
• Extended shelf life: Improved survival rates in varying heat and humidity conditions for up to six months

Technology readiness level

This technology is currently at TRL 4, with successful proof of concept demonstrated in E.coli Nissle. Future validations include engineering and testing DtpA-expressing Lactobacillus and Bifidobacterium strains, assessing compatibility with gummy manufacturing, and evaluating long-term viability and shelf stability.