Pseudotargeted analytical method for mycotoxin detection in grain extracts
A new pseudotargeted analytical method combines SWATH and mass spectrometry molecular networks to efficiently detect mycotoxins in grains with high sensitivity, throughput, and coverage.
Overview
This innovative pseudotargeted analytical method aims to revolutionize the detection of mycotoxins in grain extracts by integrating the benefits of both targeted and untargeted analytical approaches. The method employs SWATH and mass spectrometry molecular network technologies to provide comprehensive coverage and high sensitivity in identifying mycotoxins. This hybrid approach is designed to address the limitations of traditional methods, offering a more efficient solution for the analysis of diverse mycotoxins in grains.
Technical specifications
Key features:
- Combines SWATH (Sequential Window Acquisition of All Theoretical Mass Spectra) technology with molecular networking for broad spectrum analysis.
- Utilizes tandem mass spectrometry (MS/MS) to visualize and identify sets of related molecular spectra not detectable by conventional means.
- Employs MRM (Multiple Reaction Monitoring) for high-sensitivity quantitative analysis.
- Designed for high throughput and extensive coverage of various mycotoxins such as DON, Aflatoxin, Fumonisin, Ocratoxin, and Zearalenone.
Technology readiness level
The pseudotargeted analytical method has reached Technology Readiness Level 4, indicating that it has been validated in a laboratory environment with typical mycotoxin samples. Future validation steps include constructing mass spectrometric molecular networks and developing a quantitative analysis method using MRM data acquisition.
About Jiangnan University
Jiangnan University is a comprehensive public research university in Wuxi, China, known for a research-driven, industry-engaged character. State-level research platforms are co-located with pilot-scale facilities that support process development and scale-up, enabling direct collaboration with enterprises on applied R&D and technical services. A university science park, joint research institutes with regional governments, and a National Technology Transfer Center provide structured on-ramps for co-development, sponsored research, and rapid deployment. Research is supported by competitive national and provincial programs, including the National Natural Science Foundation of China and the Ministry of Science and Technology’s National Key R&D Program. Clinical partners through the university’s affiliated hospital extend capabilities into translational and human-centered studies.
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