Quantitative mycotoxin detection using LC-MS-MS
Developing a sensitive and reliable method for detecting mycotoxins in grains using isotope-labelled standards and LC-MS-MS, enhancing food safety and quality.
Overview
This research focuses on developing a quantitative method for detecting mycotoxins in grain extracts, such as deoxynivalenol and alfatoxin, using liquid chromatography tandem mass spectrometry (LC-MS-MS). By employing isotope-labelled internal standards, the method aims to provide simple, sensitive, and reliable detection, which is crucial for ensuring food safety and quality control in the agricultural industry.
Technical specifications
- Detection Targets: Deoxynivalenol, alfatoxin, fumonisin, ccratoxin, and zearalenone.
- Methodology: Utilizes isotope-labelled internal standards coupled with LC-MS-MS for precise quantification.
- Matrix Testing: Includes testing on simple matrices like cellulose and complex matrices such as cereal grains.
- Validation: Performance demonstrated through instrument and method detection limits, with the potential use of commercial certified reference materials for final validation.
Technology readiness level
Currently at TRL 3, the method is in the early stages of development with initial validation ongoing. Further testing and refinement are planned to advance through higher TRL stages, leveraging previous expertise in environmental testing and steroid hormone quantification.
About University of Nebraska, Lincoln
The University of Nebraska–Lincoln is a comprehensive public research university and the flagship campus of the University of Nebraska system, combining land-grant reach with a collaborative, industry-engaged culture. A research and technology park adjacent to campus provides modern wet and dry labs, greenhouses, offices, and conferencing, enabling companies to co-locate with faculty and access shared equipment and pilot environments. A statewide extension network links university expertise with producers and communities, creating rapid pathways for field trials, demonstrations, and workforce pipelines. Research is supported by competitive federal funding from NSF, USDA, DOE, and NIH. A dedicated technology transfer office manages IP, licensing, agreements, and startup formation with industry-friendly terms.
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