Rapid and sensitive Salmonella detection using CRISPR-Cas12 and OmniLog systems
Advanced technology utilizing CRISPR-Cas12 and OmniLog systems enables rapid, sensitive detection of Salmonella in food. By combining nucleic acid recognition with phenotypic analysis, it enhances detection accuracy and efficiency, supporting improved food safety monitoring.
Overview
The innovative solution combines the CRISPR-Cas12 system with the OmniLog phenotypic microarray system to detect Salmonella in food samples more rapidly and sensitively than traditional methods. This approach leverages CRISPR-Cas12's targeted nucleic acid recognition and fluorescence-based amplification capabilities to offer a rapid and specific detection method. By integrating OmniLog's analysis of metabolic activity and growth kinetics, the system enhances the specificity and efficiency of Salmonella detection, even in complex food matrices, ensuring compliance with food safety standards.
Technical specifications
- CRISPR-Cas12 System: Utilizes nucleic acid recognition and amplification to detect Salmonella with high specificity and sensitivity.
- OmniLog System: Analyzes metabolic responses and stress reactions of Salmonella, optimizing detection conditions.
- Combined Approach: Reduces enrichment time to 4-5 hours and uses metabolic signatures for improved detection accuracy.
- Detection Process: The Cas12-crRNA complex targets specific DNA sequences, cleaves fluorescent probes, and generates detectable signals, minimizing false negatives.
Technology readiness level
This technology is currently at Technology Readiness Level 3, indicating that it has been validated in a laboratory setting. Future validation plans include optimizing growth conditions and refining detection methods to enhance reliability and reduce assay time.
About AgResearch
AgResearch is a New Zealand government research organization focused on the agricultural sector; formerly an independent Crown Research Institute, since July 1, 2025 it has operated as a group within the Bioeconomy Science Institute. With major campuses co‑located at Lincoln University and near Massey University, and shared spaces with FoodHQ partners, industry teams can access specialized labs and collaborative project space. Company‑owned research farms nationwide enable field validation and scale‑up alongside commercial partners. Research is supported by New Zealand Government investment through MBIE programmes such as the Strategic Science Investment Fund and National Science Challenges, complemented by competitive grants and contract research. A dedicated commercialisation team manages IP, patents, licensing, and MVP development, and connects through national commercialisation networks.
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