Rapid biosensor for detecting Salmonella in chocolate using DNAzyme technology
Introducing a novel DNAzyme-based electrochemical biosensor, DNAlyzer, for rapid, sensitive, and specific detection of Salmonella in chocolate. This microfluidic system utilizes RNA-cleaving DNAzymes to identify the RNase H2 enzyme, offering a culture-free detection method.
Overview
The DNAlyzer is an innovative DNAzyme-based electrochemical biosensor designed for rapid detection of Salmonella in chocolate matrices. By utilizing RNA-cleaving DNAzymes (RCDs) that target the RNase H2 enzyme, conserved across all Salmonella species, this biosensor provides a sensitive and specific test. The integration of a microfluidic system facilitates the detection process by flowing diluted and heated chocolate through the sensor, overcoming mass transport limitations and eliminating the need for growth cultures.
Technical specifications
- Functional nucleic acids (RNA-cleaving DNAzymes): Engineered to detect Salmonella's RNase H2 enzyme and release a DNA barcode for identification.
- Microfluidic system: Comprises a biorecognition zone with microgel magnetic beads modified with Salmonella RCDs and a detection zone where DNA barcodes generate an electrochemical signal.
- Sample preparation: Involves dilution with vegetable oil and temperature-controlled flow analysis to optimize detection in chocolate.
- Limit-of-detection: Capable of detecting as low as 1 CFU in ≤4 hours, with tested validation in various food matrices including milk, egg, beef, and chicken.
Technology readiness level
Currently at TRL 4, the DNAlyzer has demonstrated proof-of-concept functionality in laboratory settings with plans for further development and optimization, including systems integration and performance testing in chocolate matrices.
About McMaster University
McMaster University is a comprehensive, research‑intensive public university in Hamilton, Ontario, known for collaborative, problem‑driven scholarship and strong partnerships with healthcare and industry. A research and technology park adjacent to campus co‑locates corporate R&D with faculty labs, while an established engineering co‑op connects companies with talent and applied expertise. Deep integration with regional hospital systems enables clinical trials, real‑world evidence generation, and translational studies at scale. Research is supported by competitive funding from NSERC, CIHR, SSHRC, and the Canada Foundation for Innovation. A dedicated technology transfer office streamlines IP strategy, contracting, and commercialization for industry partners.
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