Rapid chicken bioassay for determining caloric value of reduced-fat foods and modified oils

Facilities & testing
University

A 48-hour chicken bioassay that rapidly determines the caloric value and nutrient digestibility of foods, feed ingredients, and modified fats and oils. Proven across 4,000+ samples, it provides an efficient model for quantifying caloric reductions in low-fat potato chips and oils engineered for reduced intestinal absorption.

Overview

This solution offers a rapid 48-hour chicken bioassay for determining the caloric value and nutrient digestibility of foods, feed ingredients, and lipid products. Developed and refined at the University of Illinois Urbana-Champaign, the assay provides a fast and reliable method for quantifying caloric content in reduced-fat food products such as low-fat potato chips, as well as modified fats and oils designed to minimize intestinal absorption. With a track record spanning thousands of tested samples across human foods, companion animal diets, zoo animal nutrition, and industrial byproducts, the bioassay serves as a practical model for human caloric evaluation and supports product development for food manufacturers and ingredient innovators.

Technical specifications
  • 48-hour rapid bioassay delivering caloric value and digestibility results in a fraction of the time required by traditional methods
  • Gavage-based delivery allowing evaluation of virtually any food, ingredient, or modified lipid regardless of form or palatability
  • Broad applicability across carbohydrates, lipids, proteins, amino acids, and complex feed ingredients
  • Validated across species including humans, food animals, companion animals, and zoo animals
  • Extensively published methodology with hundreds of peer-reviewed papers supporting the approach
  • Proven sample diversity covering reduced-calorie carbohydrates like polydextrose, maltodextrins, and resistant starches, as well as ethanol industry byproducts, algae, and microbial biomass
  • Quantitative caloric reduction assessment for modified oils engineered to reduce intestinal absorption
Technology readiness level

The bioassay is fully developed and validated, having been used to determine nutrient digestibility for more than 4,000 different food and feed ingredient samples across numerous species. Hundreds of peer-reviewed publications document its reliability and reproducibility. The assay is immediately available for collaborative application to low-fat potato chip products and modified fat and oil ingredients, with future validation planned through partnerships with laboratories developing reduced-calorie snack products and novel lipid modifications. The technology is ready for deployment in sponsored research or fee-for-service arrangements with industry partners seeking rapid, quantitative caloric assessments.


About University of Illinois, Urbana-Champaign

The University of Illinois Urbana‑Champaign is a flagship public research university with large‑scale research capacity and a broad academic portfolio. An on‑campus Research Park co‑locates corporate R&D teams and startups with faculty, while the National Center for Supercomputing Applications provides advanced computing and data capabilities for collaboration. Integration with a regional health system and an engineering‑based college of medicine enables clinical translation, and a long‑standing extension network links campus innovation to partners statewide. Research is supported by competitive federal funding from NSF, NIH, DOE, USDA, and DoD. A technology transfer office streamlines IP, licensing, and startups, complemented by incubators and prototyping in the Research Park.

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