Rapid on-site detection kit for Salmonella in food matrices
A SHERLOCK-based rapid detection kit for Salmonella, designed for the chocolate industry, offering reliable, sensitive on-site testing to manage food safety risks.
Overview
The University of Tasmania is developing a rapid detection kit for Salmonella spp. using the SHERLOCK system, originally designed for tumor cell detection. This innovative kit aims to provide the chocolate industry with an efficient, reliable, and sensitive tool for managing food safety risks associated with Salmonella contamination in products like cocoa and chocolate.
Technical specifications
The detection kit utilizes the SHERLOCK system, which includes:
- Recombinase Polymerase Amplification (RPA): Amplifies target DNA followed by secondary amplification and conversion to RNA.
- CRISPR-mediated detection: Utilizes CRISPR to cleave reporter probes, providing a clear visual signal.
- One-pot system: Ensures efficiency and suitability for on-site applications, allowing results within 60 minutes.
- Lateral flow-based visual readout: Adapted from a COVID-19 diagnostic kit, enabling easy and rapid interpretation of results.
Technology readiness level
This technology is currently at a TRL of 4, indicating that the core components are validated in a laboratory environment. The project aims to develop a prototype detection system within one year, ready for scale-up and commercial use.
About University of Tasmania
The University of Tasmania is a comprehensive public research university with a statewide footprint across Hobart, Launceston and Burnie. Industry connects through co‑located assets including the Australian Maritime College’s hydrodynamics and simulation facilities and the Institute for Marine and Antarctic Studies in Hobart’s Antarctic gateway, plus clinical schools embedded in state hospitals. An Industry 4.0 Integrity of Food Testlab and the Launceston Institute help SMEs trial digital technologies and collaborate with university expertise, while the Tasmanian Institute of Agriculture runs research farms that enable scale‑up and extension. Research is supported by competitive funding from the Australian Research Council and the National Health and Medical Research Council. Commercialisation is delivered by InVent (Innovation Ventures), providing IP management, licensing and startup pathways.
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