Rapid salmonella detection using LAMP assay with lateral flow readout
A novel LAMP-LFA assay for rapid and sensitive detection of Salmonella enterica. This method combines DNA amplification with a lateral flow readout, offering minimal labor, time, and equipment requirements. It is suitable for complex matrices like food products.
Overview
The University of Lincoln presents a cutting-edge solution for the rapid detection of Salmonella enterica using a Loop-Mediated Isothermal Amplification (LAMP) assay with a lateral flow assay (LFA) readout. This innovative method is designed to deliver exceptional sensitivity and reduce labor and time requirements. The assay is based on specific LAMP primers and universal dipsticks that are compatible with conventional primer labeling. It includes a conjugate control to verify LFA dipstick functionality and a designer amplification control to ensure correct amplification conditions, significantly minimizing the risk of false negatives. This approach is particularly suitable for complex matrices such as processed foods, chocolate, cereals, and meat.
Technical specifications
- LAMP-LFA method: Combines DNA amplification with a lateral flow readout, making it easy and portable.
- Minimal equipment needed: Only a heat block and a small benchtop centrifuge are required.
- Optional PMA use: The method can include photo-reactive DNA-binding dyes to remove DNA from non-viable cells, focusing on viable cell amplification.
- Compatibility: Works with a commercial DNA extraction kit that requires minimal handling, suitable for complex food matrices.
- Internal controls: Incorporates amplification controls to reduce false negatives.
Technology readiness level
Currently at TRL 4, this technology has been validated in a laboratory environment. Future work packages will focus on optimizing the assay for Salmonella enterica, verifying specificity, assessing analytic performance, and validating the method against existing standards with actual samples. This will ensure that the assay meets high diagnostic performance criteria, aiming for a minimum sensitivity and specificity of 90% with rigorous testing protocols.
About University of Lincoln
The University of Lincoln is a comprehensive public university with a modern, city-centre campus and an applied research culture. Industry engagement is built into its model through co-located facilities at a dedicated science and innovation park and long-term partnerships with global manufacturers, utilities, and regional SMEs. Specialized food manufacturing and packaging testbeds at its south Lincolnshire campus provide pilot-scale lines for process development, while links with regional NHS trusts and a medical school enable clinical research and evaluation. Research draws competitive support from UKRI councils, Innovate UK, and the National Institute for Health and Care Research, alongside partnerships with industry. A dedicated technology transfer and enterprise office supports IP management, licensing, and spinout formation.
Inquire about this partnering listing
Industry applications
delivered to your inbox.