Recyclable yeast with surface-displayed decarboxylase for direct alpha olefin conversion

Overview

This innovative solution involves the use of genetically modified yeast cells, specifically Pichia pastoris, to express a cofactor-free decarboxylase enzyme on their surface. This enzyme, OleT (CYP152) decarboxylase, enables the direct conversion of fatty acid methyl esters (FAMEs) into linear alpha olefins, a valuable chemical feedstock. The yeast cells can be easily recycled through centrifugation or filtration, making the process not only efficient and cost-effective but also sustainable. This technology is particularly beneficial for industries looking to optimize the production of bio-based olefins with reduced environmental impact.

Technical specifications

• Cofactor-free decarboxylase expression: The enzyme OleT (CYP152) is displayed on the yeast cell surface, eliminating the need for additional cofactors.
• High productivity: Genetic modifications allow for enzyme activity levels reaching 200 g/L.h.
• Robust recycling: Yeast cells can be recycled up to ten times without significant loss of enzyme activity.
• Versatile applications: Co-expression of lipase for simultaneous conversion of triglycerides on the same yeast cells is possible.

Technology readiness level

Currently at TRL 6, the technology has been demonstrated in a relevant environment. Future validation steps include improving enzyme activity through genomic integration and testing with commercial-grade fatty acid methyl esters and triglycerides. The process is poised for further development towards industrial application.