Scalable mono-hydroxylation process using peroxygenases
GECCO-Biotech has developed a scalable, cost-effective mono-hydroxylation process using peroxygenases. This green technology provides efficient C-H bond activation under mild conditions, reducing synthetic steps and costs for industrial applications.
Overview
GECCO-Biotech presents an innovative mono-hydroxylation process leveraging the capabilities of peroxygenases, a type of oxidoreductase enzyme. These enzymes enable selective oxidation reactions, specifically C-H bond activation for hydroxylation, under mild and environmentally friendly conditions. This technology is poised to transform industrial chemical synthesis by offering a scalable and cost-effective alternative to traditional methods, which often require multiple steps, expensive reagents, and prolonged development times.
Technical specifications
Key features:
- Utilizes peroxygenases for efficient hydroxylation reactions
- Operates under mild conditions with hydrogen peroxide as the sole oxidant
- Environmentally friendly and reduces the need for multiple synthetic steps
- Capable of being scaled from gram-scale to kg-scale production
- Demonstrated enzyme production scale-up using E. coli, which is more cost-effective than eukaryotic systems
Development process:
- Phase 1 involves screening substrates with available peroxygenases to optimize activity
- Phase 2 focuses on biocatalytic process development, including enzyme immobilization and reactor optimization
- Emphasis on downstream process development to isolate products without contaminants
Technology readiness level
This technology is currently at Technology Readiness Level 4, indicating that components have been validated in a laboratory environment. Ongoing efforts include further process optimization and scale-up to achieve commercial viability.
About GECCO-Biotech
GECCO Biotech is a biotechnology research company that specializes in enzyme-driven technologies, providing comprehensive support for industrial applications in sectors such as pharmaceuticals, flavors, fragrances, and food technology. The company acts as a one-stop shop for biocatalysis, offering services that bridge the gap between fundamental enzyme research and practical industrial implementation. Their technical capabilities include enzyme scouting, custom cloning and expression, high-throughput enzyme engineering to improve performance metrics like stability and selectivity, and process optimization to refine reaction parameters such as pH, temperature, and substrate concentration. GECCO also provides ready-to-use enzyme kits, containing purified enzymes in well-plate formats to streamline substrate screening and reaction analysis.
By helping organizations integrate enzymatic solutions into their production pipelines, GECCO enables the transition toward more sustainable, efficient, and environmentally friendly manufacturing processes. Their team of experts, largely holding PhDs, collaborates with international research centers and industrial partners on high-impact R&D projects to solve complex biocatalytic challenges. The company's work, which includes the development of proprietary enzyme technologies and advanced analytical testing, supports clients in achieving specific milestone targets for product development, often resulting in enhanced cost and environmental efficiency. GECCO operates from its headquarters in Groningen, Netherlands, and is deeply involved in various EU-funded initiatives focused on next-generation enzymes and bio-based industrial production.
delivered to your inbox.