Selective amide synthesis from crude glycerine for purification and cost reduction
Innovative process using immobilized protease biocatalyst to selectively synthesize amides from crude glycerine fatty acids, enhancing purification and reducing costs. Suitable for industrial glycerine processing with efficient recycling of components.
Overview
This innovative process leverages a highly selective biocatalyst, immobilized protease, to convert fatty acids in crude glycerine into amides. The resulting amides, characterized by high melting points, can be easily removed through filtration, enhancing the purification of glycerine and significantly reducing production costs. This method is particularly beneficial for industrial glycerine processing, offering an efficient route to utilize crude glycerine by-products while maintaining high glycerine yield.
Technical specifications
- The process begins with a mixture of crude glycerine and gaseous ammonia or short-chain amines like ethylamine.
- Immobilized proteases facilitate the conversion of fatty acids into amides, without affecting the glycerine yield.
- The selective synthesis relies on enzymes' high specificity, transforming fatty acids and esters into amides.
- Produced amides can be removed via filtration due to their high melting points, with ammonia and ethylamine being recycled to minimize waste.
- Validation involves testing various commercial proteases, using real sample mixtures in pressurized environments, and optimizing conditions through filtration and gas chromatography.
Technology readiness level
Currently at TRL 4, this process has been validated in laboratory settings with commercial triglycerides and pure fatty acids. Future plans include optimizing protease selection and scaling the process to industrial settings.
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A specialized public university in Wrocław focused on environmental and life sciences, recognized for applied, industry‑engaged research. Companies collaborate through on‑site laboratories, field research sites, controlled‑environment facilities, and veterinary clinical services that enable testing, validation, and demonstration with academic experts. Its location within Wrocław’s innovation ecosystem and the broader Lower Silesia economy supports contract studies, field trials, and workforce pipelines with regional producers, utilities, and technology firms. Research is supported by competitive European and national programs, including EU framework funding and Poland’s principal science and innovation agencies. A central technology transfer office manages IP, licensing, and startup formation while providing a single front door for industry.
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