Single-cell RNA sequencing for cell-type specific expression analysis
SolusCell offers high throughput single-cell RNA-sequencing services to elucidate the impact of promoter SNPs on gene expression at the cell-type level, facilitating functional genetic variant characterization in agriculture.
Overview
SolusCell provides a specialized service utilizing high throughput single-cell RNA-sequencing (scRNA-seq) to explore the connection between promoter SNP variations and their effects on gene expression at a cell-type specific level. This service is pivotal for characterizing genetic variants, identifying their influence on gene expression across diverse cell types, and understanding downstream functional effects. It addresses the limitations of traditional tissue-wide approaches, offering precise insights into gene expression changes within specific cell types.
Technical specifications
- Service process: Includes nuclei isolation, library preparation, sequencing, and bioinformatics analysis, all completed within 4-6 weeks.
- Data analysis: Sequencing reads exceed 250 million per sample, aligned to a client-selected reference genome. Results include cell type-specific expression patterns, with statistical analysis to detect genotype-specific changes.
- Quality control: Ensures high-quality data through rigorous processes, eliminating empty cells and potential doublets, and integrating data with control genotypes for comprehensive analysis.
Technology readiness level
This service is fully operational and validated, with a Technology Readiness Level of 9, indicating readiness for full-scale deployment in industrial and academic research settings.
About SolusCell LLC
SolusCell provides specialized single-cell genomic services focused on plants, animals, and fungi. The company offers a tiered range of services, including scBASIC for nuclei isolation and library preparation, scPLUS for sequencing and preliminary bioinformatics using their proprietary SolusCell Annotator database, and scPREMIUM for long-term data mining and storage. By optimizing complex nuclei isolation protocols, they address the challenges associated with difficult-to-dissociate tissues, enabling researchers to obtain high-quality genomic insights regardless of the organism.
These services are designed to democratize access to single-cell genomic technologies, supporting researchers who require specialized technical and analytical expertise to explore cellular heterogeneity and trait regulation. The company assists clients in addressing critical biological questions, such as environmental responses and developmental processes, by providing accessible data analysis and interpretation tools. Founded by a team with extensive experience in genomics, bioinformatics, and R&D, SolusCell operates from its headquarters in Alachua, Florida, to facilitate scientific discovery across diverse academic and industrial research environments.
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