Single-tube multiplex SNP amplification and sequencing-ready library preparation
A cutting-edge single-tube, one-step solution for multiplex SNP amplification and sequencing-ready library preparation, allowing scalability and efficient genotyping. Suitable for Illumina and AVITI platforms, it simplifies the process with minimal hands-on time.
Overview
This innovative solution provides a single-tube, one-step method for multiplex SNP amplification and sequencing-ready library preparation. The process requires minimal hands-on time, enabling users to prepare a sequencing-ready library by simply adding template DNA, target SNP primers, and PCR reagents to a PCR plate. The technology supports scalability with 384 sample indexing primers and barcoded primer pool sets, allowing the determination of 10-100 SNPs per reaction and capable of processing up to 384,000 genotypes with ease. The resulting library can be sequenced using platforms like Illumina's NextSeq 2000.
Technical specifications
- Hands-on time: Approximately 20 minutes for full plate assembly.
- Scalability: Supports 96 or 384 sample indices, with barcoded primer pool sets.
- Throughput: Capable of determining 10-100 SNPs per reaction.
- Sequencing platforms: Compatible with Illumina and AVITI platforms.
- Software: Includes cloud-based or local processing for genotype determination.
- Sample compatibility: Evidence of direct PCR compatibility with saliva or cheek swabs, with an inhibitor-tolerant PCR mastermix.
Technology readiness level
The technology is at TRL 9, indicating it is fully developed and commercially available. It is currently utilized in products such as the Human Sample ID kit and mini kit, demonstrating its market readiness and proven effectiveness.
About pxlence
pxlence is a Ghent University spin-off established in 2015 that specializes in the development and manufacturing of PCR-based assays and kits for targeted resequencing and nucleic acid quantification. The company offers three core product lines: a catalogue of nearly one million predesigned PCR assays for targeted resequencing of human exonic regions; universal Rainbow DNA detection probes designed for digital PCR and quantitative PCR to streamline multiplexing; and Human Sample ID kits used for DNA authentication and sample tracking. Their technology centers on a proprietary high-throughput primer design pipeline that allows for high-performance amplification and precise genetic targeting across various sample types, including genomic DNA, FFPE, and cfDNA.
These solutions are primarily utilized by clinical diagnostic laboratories and medical device companies to address critical needs in molecular diagnostics and precision medicine. By providing standardized, high-quality assays that integrate with NGS workflows and digital PCR platforms, pxlence helps researchers and diagnostic professionals improve accuracy, reduce costs, and simplify experimental design. The company has demonstrated the efficacy of its tools through peer-reviewed research and collaborative projects aimed at advancing cancer detection and genetic variant analysis, supporting the broader healthcare industry in enhancing diagnostic reliability and throughput.
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