Solvent-free microbial lipid recovery via two-phase fermentation
Innovative two-phase fermentation system for solvent-free microbial lipid recovery, reducing energy use and simplifying downstream processing. This method employs a biocompatible carrier phase for in-situ lipid partitioning, enhancing efficiency and scalability.
Overview
The two-phase fermentation system offers an innovative approach to microbial lipid recovery by enabling in-situ extraction during fermentation. By introducing a biocompatible lipid carrier phase into the fermentation broth, lipids are directly partitioned into this oil phase, which significantly reduces the need for traditional energy-intensive downstream processes. This method not only simplifies the recovery process but also minimizes solvent use, making it an environmentally friendly alternative. The technology promises improved lipid recovery efficiency, reduced process complexity, and greater alignment with sustainable industrial practices.
Technical specifications
Key features:
- Utilizes a biocompatible lipid carrier phase for in-situ lipid partitioning
- Reduces the need for solvent extraction and energy-intensive downstream processes
- Combines hybrid separation techniques such as centrifugation and membrane filtration to isolate lipid-rich phases
- Potentially applicable to various industrial fermentation systems for producing lipid-soluble metabolites
Process Steps:
- System design and screening: Selection of suitable lipid carrier phases and evaluation of lipid partitioning behavior.
- Membrane clarification: Efficient isolation of lipid-rich phases.
- Recovery and purification: Assessment of lipid recovery efficiency, product purity, and energy requirements.
- Process evaluation: Mass balances, recovery yields, and techno-economic analysis to determine scalability and industrial feasibility.
Technology readiness level
This technology is currently at TRL 4, indicating that it has been validated in lab settings but requires further development and scaling to reach commercial readiness. The ongoing research aims to generate proof-of-concept data and process metrics to support its integration into industrial systems.
About Colorado State University
Colorado State University is a comprehensive public land‑grant research university with an applied, partnership‑driven culture. Multiple research campuses—including the Fort Collins main campus, a public‑facing Denver site, and a foothills research complex with shared core facilities and pilot‑scale testbeds—enable companies to co‑locate, access instrumentation, and run validation studies. A statewide Extension network and proximity to the Front Range innovation corridor provide streamlined engagement with regional and national industry, while an integrated veterinary teaching hospital supports translational studies. Research is supported by competitive federal funding from agencies such as NSF, NIH, USDA, DOE, and DoD. A dedicated technology transfer office streamlines IP, contracting, and startup formation, with incubator and collaboration space for industry partners.
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