Transcriptomic signature analysis of neural progenitor cell senescence
This research explores the relationship between mitochondrial ROS production and cellular ageing in neural progenitor cells derived from Parkinson's disease patients. By identifying transcriptomic signatures, it aims to uncover therapeutic targets for PD and ageing.
Overview
This research focuses on understanding the interplay between mitochondrial reactive oxygen species (mtROS) production and cellular ageing in neural progenitor cells (NPCs) derived from induced pluripotent stem cells (IPSCs) of Parkinson's disease (PD) patients. By performing RNA sequencing on NPCs with varying levels of mtROS, the study seeks to identify transcriptomic signatures that could reveal new therapeutic targets for PD and general ageing processes.
Technical specifications
- Objective: To study the effect of mtROS on cellular senescence in NPCs and its potential link to Parkinson's disease.
- Methods:
- Induction of cellular senescence using irradiation and RecQ DNA helicase knockout.
- Separation of NPC populations based on mtROS levels using flow cytometry and MitoSox dye.
- RNA sequencing of sorted cell populations to identify transcriptomic differences.
- Potential Applications: Identification of therapeutic targets for PD and insights into the ageing process.
Technology readiness level
This research is at Technology Readiness Level 4, indicating that the concept has been validated in a laboratory environment through the use of positive control senescent cells and mtROS profiling techniques.
About University of Tübingen
Eberhard Karls University of Tübingen is a large, comprehensive public research university in Baden‑Württemberg with a global outlook and a strong record of basic and translational discovery. Its integration with a major university hospital supports clinical research and translation at scale. Co‑located national research institutes and a vibrant regional innovation ecosystem enable joint labs, shared facilities, and industry‑sponsored collaborations, while proximity to Stuttgart’s manufacturing and mobility hub facilitates pilot testing and scale‑up. Research is supported by competitive funding from the German Research Foundation, the Federal Ministry of Education and Research, and European Union programs. A dedicated technology transfer office manages IP, licensing, startup formation, and streamlined contracting for corporate R&D.
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