Ultrasonic elastography for non-invasive embryo ploidy determination
A novel non-destructive ultrasonic elastography method for sorting embryos based on ploidy, offering a safe alternative to traditional invasive techniques. Utilizes effective bulk modulus elastography (EBME) to differentiate between diploid and haploid embryos with high precision.
Overview
The novel ultrasonic elastography technology provides a revolutionary method for the early-stage determination of embryo ploidy in a non-destructive manner. This solution offers a significant advancement over traditional methods, such as fluorescent staining, which can be lethal. Utilizing effective bulk modulus elastography (EBME), this technology measures the mechanical properties of embryos, allowing for precise differentiation between diploid (2N) and haploid (1N) embryos based on density and bulk modulus. This method preserves embryo viability, enabling downstream regeneration and high-throughput testing.
Technical specifications
The EBME system operates by mapping embryo mechanical properties through the emission of a monostatic ultrasonic pulse. This pulse measures acoustic impedance and the speed of sound, leading to calculations of effective density and bulk modulus without requiring external compressive stress. The integrated endoscopic EBME system uses an optical endoscope to locate embryos, with a co-registered ultrasonic transducer performing measurements. Key features include:
- Non-destructive, label-free acoustic sorting
- High precision localization and mapping of 500-micron targets
- Resolution of spatial separations as small as 24 microns
Technology readiness level
This technology is currently at TRL 4, indicating that it has been validated in a laboratory environment. The development plan includes four phases to transition to a high-throughput embryo sorting platform, aiming to optimize system components, establish baseline mechanical thresholds, automate classification, and validate sorting accuracy through blind trials.
About University of North Texas
The University of North Texas is a comprehensive public research university in Denton, part of the UNT System, serving a large and diverse student body with broad academic programs. A dedicated research campus brings engineering and science together with shared user facilities, advanced instrumentation, prototyping spaces, and technology transfer support that speed collaboration with industry. Its Dallas–Fort Worth location offers ready access to major corporate R&D, suppliers, and testing partners, supported by project-based engagements, internships, and sponsored capstones. Research is backed by competitive federal funding from agencies such as the National Science Foundation, Department of Defense, and Department of Energy, alongside state and industry support.
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