Understanding cardiomyopathy mechanisms in progeroid syndromes using ipscs
This research leverages patient-derived induced pluripotent stem cells (iPSCs) to study the mechanisms leading to dilated cardiomyopathy (DCM) in Hutchinson-Gilford progeria syndrome (HGPS). The integrative model aims to identify disrupted pathways, providing insights into both HGPS and age-related DCM.
Overview
This research initiative focuses on understanding the underlying mechanisms of dilated cardiomyopathy (DCM) in Hutchinson-Gilford progeria syndrome (HGPS) using induced pluripotent stem cells (iPSCs) derived from patients. By analyzing these cells, researchers aim to uncover the pathways that lead to premature cardiac dysfunction. This study not only targets the mechanisms within HGPS but also offers insights into DCM that occurs due to normal aging, potentially guiding novel therapeutic approaches.
Technical specifications
- Utilizes a biobank of normal and HGPS patient-derived iPSCs.
- Validated cardiac differentiation protocols to model cardiomyopathy.
- Multiomic analyses, including RNA-seq and Repli-seq, to detect gene expression changes.
- Integration of gene regulatory network models to identify disrupted pathways.
- Evaluation of cell proliferation, senescence, and DNA damage markers using flow cytometry, SA-B-Gal staining, and gH2AX immunofluorescence.
Technology readiness level
The research is currently at Technology Readiness Level 3, indicating that experimental proof of concept has been demonstrated through preliminary studies and validation of iPSC differentiation protocols and gene regulatory network models.
About University of Minnesota
The University of Minnesota is a flagship, comprehensive public research university spanning multiple campuses, with a large research enterprise and clinical integration. Industry engages through co-located labs on the Twin Cities campuses, access to an academic health system for clinical translation, and pilot and field-testing facilities that speed scale-up. A statewide extension network and outreach centers provide real-world sites and data partnerships across Minnesota, while proximity to a dense medtech and Fortune 500 corridor enables frequent collaboration. Research is supported by competitive federal funding, including NIH, NSF, DOE, USDA, and DoD. A dedicated technology transfer office manages IP, licensing, sponsored research agreements, and startup incubation to speed commercialization.
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