Exploring wheat rhizoplane bacteria to enhance resistance to root-knot nematodes, leveraging plant growth-promoting bacteria as biocontrol agents. Promising strains show up to 90% nematicidal activity, offering sustainable disease management in agriculture.
This groundbreaking research explores the use of wheat rhizoplane bacteria as a sustainable solution for enhancing plant resistance against root-knot nematodes (RKNs). The study focuses on plant growth-promoting bacteria (PGPB) isolated from Triticum aestivum, which are environmentally friendly agents capable of suppressing nematodes while enhancing plant health. From a collection of over 300 isolates, certain strains have been identified with genomic signatures indicative of nematicidal activity. These bacteria provide a dual benefit by targeting nematodes and promoting plant growth, aligning with sustainable agriculture needs.
The research identified bacterial strains with nematicidal properties, confirmed through in vitro testing of cell-free culture supernatants, both crude and filtered. Crude supernatants demonstrated up to 90% nematicidal activity, while filtered fractions retained 50-60% efficacy, indicating the presence of stable bioactive compounds. The ongoing assays in Arabidopsis aim to further validate these strains by assessing their ability to reduce nematode infectivity. The future steps include greenhouse evaluations in tomatoes to test biocontrol efficacy and plant growth promotion.
This innovative approach is currently at Technology Readiness Level 3. Initial in vitro validations have been successful, with plans for further testing in controlled environments and greenhouse settings to advance the development of these biocontrol agents.
Universidad de Salamanca is a large, comprehensive public research university with a historic campus and a multi-campus footprint across western Spain. Industry collaborates through a research and technology park that co-locates laboratories, prototyping spaces, and incubator offices near campus. Integration with the regional health system and the university hospital enables clinical research, while structured internship and contract-research programs make it easy to engage faculty and student talent. Research is supported by competitive national funding and European programs, including Horizon Europe. A dedicated technology transfer office streamlines IP management, licensing, and startup formation, connecting partners across Spain and the EU.