Automated dna-based detection of known target sequences using LAMP technology

Overview

This technology is an automated, portable DNA detection platform designed to identify known target sequences—such as genetic modifications in crops—directly from crude samples like ground seeds or leaves. The system integrates a sample processing step, a self-contained LAMP (loop-mediated isothermal amplification) assay cassette, and an optical or electrochemical readout to deliver a clear yes/no result without the need for a laboratory setting.

The core value proposition is enabling rapid, user-friendly, on-site DNA detection for agricultural, regulatory, and biosecurity applications. By automating tissue processing and reagent handling, the device reduces the need for skilled operators and shortens the time from sample collection to result. Potential users include seed producers, regulatory bodies inspecting for genetically modified organisms, agricultural supply chain stakeholders, and plant health professionals monitoring for pathogens or specific traits.

Technical specifications

Key features:

• Automated sample capture and processing – The device captures particulates or processes tissue material, releases DNA, and performs a filtration step to prepare the sample for amplification.
• LAMP assay cassettes – Self-contained cassettes contain freeze-dried, stable primers specific to a target DNA sequence (e.g., a genetic modification), eliminating the need for cold-chain reagent storage. Current stability is approximately two months at 40 °C.
• Isothermal amplification and detection – The reaction is heated to the required temperature and the amplification product is measured via fluorescence or electrochemistry, producing a clear result.
• Wireless data communication – Results are transmitted wirelessly to a server for logging, remote access, and integration with existing data platforms.
• Miniaturised microfluidic design – The process is being refined into a compact, user-friendly format with an LCD or touchscreen display for direct result viewing.

The platform builds on prior collaborative work with UK-based SMEs to develop automated airborne spore detection using trait-specific LAMP assays, extending the approach to GMO detection and other sequence-based targets.

Technology readiness level

The underlying components have been demonstrated in a fully automated device capable of capturing airborne particulates, releasing DNA, filtering, amplifying with freeze-dried reagents, and reading fluorescence results wirelessly. Current development focuses on miniaturisation, improving reagent stability, designing a simple homogenisation device for tough materials, and integrating the workflow into a microfluidic cassette with a user-friendly interface. Remaining validation steps include designing and testing LAMP primers for specific GM traits, producing and stabilising freeze-dried reagent cassettes, miniaturising the fluidic system, and validating the complete prototype with seeds or leaf material spiked with synthetic or actual GM target DNA.