Botanical biopesticides from African marigold and lemongrass for fall armyworm control

Technology
Conceptual
University

Phytochemical extracts from Tagetes erecta and Cymbopogon citratus show strong larvicidal activity against Spodoptera frugiperda (fall armyworm). Preliminary data demonstrates 60% larval mortality and significant growth stagnation. Seeking partnership to fund formulation development and expanded validation.

Overview

Fall armyworm (Spodoptera frugiperda) is a destructive agricultural pest threatening crop yields across multiple regions. This research explores the use of plant-derived phytochemicals from African marigold (Tagetes erecta) and lemongrass (Cymbopogon citratus) as effective biopesticide alternatives to synthetic insecticides. Preliminary laboratory results demonstrate that ethyl acetate leaf extracts from these plants trigger significant larval growth stagnation and mortality in third instar S. frugiperda larvae, offering a promising natural pest management solution.

Technical specifications

Key findings from preliminary validation:

  • Ethyl acetate leaf extracts of T. erecta and C. citratus caused 60% larval mortality by day 4 and day 7, respectively
  • Treated larvae were significantly smaller at day 10 compared to untreated controls
  • Extracts were prepared by crushing dry leaves, adding ethyl acetate solvent, filtering, and concentrating the supernatant
  • Bioassays used laboratory-bred third instar S. frugiperda larvae (n=10 per treatment)

Planned expanded validation:

  • Replicated studies with larger sample sizes across multiple larval instars
  • Testing additional solvents (hexane, ethyl acetate, ethanol) to isolate a broader range of phytochemicals
  • Assessment of antifeedant effects, larval and pupal weight, and developmental impacts
  • Development and testing of effective extract formulations
Technology readiness level

This research is currently at an early-to-mid stage of development. Preliminary laboratory bioassays have demonstrated proof-of-concept efficacy of botanical extracts against fall armyworm larvae. The next phase requires expanded replicated trials, broader phytochemical screening across multiple solvents, and formulation development to advance toward field-ready biopesticide products. The research team is seeking funding partnership to support key personnel and formulation testing activities over a one-year validation period.


About International Centre for Genetic Engineering and Biotechnology

The International Centre for Genetic Engineering and Biotechnology is an intergovernmental, non-profit research and training organization operating laboratories in Trieste, New Delhi, and Cape Town. Its campuses provide co-located labs and shared core facilities that enable collaborative R&D, while training and fellowship programs create sustained talent pipelines for partners. Proximity to innovation parks and leading universities at each site, plus biosafety and policy advisory services, helps industry navigate translation and regulatory contexts across regions. Research is supported by member-state contributions and competitive grants from national and international agencies. A dedicated technology transfer office manages IP, licensing, and commercialization across sites.

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