CSIR - Central Scientific Instruments Organisation

Botrytis cinerea laccase enzyme system for textile stain bleaching

Technology
Conceptual
University

A novel enzyme system utilizing Botrytis cinerea laccase for environmentally friendly bleaching of polyphenol-based stains on textiles. This method operates at ambient conditions, preserving fabric integrity and color without harsh chemicals.

Overview

The Botrytis cinerea laccase enzyme system offers an innovative solution for the textile industry by providing an environmentally friendly method for bleaching polyphenol-based stains on cotton and synthetic fabrics. This enzymatic process functions under ambient temperature and pressure, eliminating the need for harsh chemicals such as sodium hypochlorite or hydrogen peroxide. As a result, it preserves the integrity and color of the fabric, making it a sustainable option for manufacturers and consumers alike.

Technical specifications
  • Enzyme Source: Botrytis cinerea, a mesophilic species known for producing diverse laccase isoforms.
  • Reaction Conditions: Operates efficiently under ambient temperature and pressure.
  • Substrate Degradation: Capable of breaking down polyphenol-based stains.
  • Cost Efficiency: Low-cost enzyme production using inducers like Gallic acid.
  • Potential Modifications: Genetic engineering to optimize pH and enhance enzyme production.
  • By-products: Generates water, supporting environmental sustainability.
Technology readiness level

Currently, the technology is at TRL 2. Further validation involves isolating and purifying enzyme isozymes, characterizing their operational limits, and optimizing their functional profiles through genetic modifications if necessary. Intensive characterization and testing of the enzyme's catalytic efficiency and stability in formulations are planned for future development.

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