Dairy cow mastitis challenge model for non-lactating holstein cows
A research model to study mastitis in non-lactating Holstein cows using controlled bacterial challenges. The model aims to determine optimal bacterial doses and study transcriptional changes in mammary gland tissues.
Overview
The Dairy Cow Mastitis Challenge Model is designed to investigate mastitis in non-lactating Holstein cows by introducing controlled bacterial challenges. This model focuses on understanding the disease's progression and identifying optimal bacterial doses for inducing mastitis. By studying clinical symptoms and pathological changes in mammary tissues, the research aims to provide insights into mastitis management and treatment in dairy cows.
Technical specifications
- Selection of Subjects: 45 three-year-old Holstein cows in good health during their dry period.
- Trial Design: Cows are divided into control and experimental groups exposed to different doses of Escherichia coli and Staphylococcus aureus.
- Bacterial Doses: Bacteria are diluted to concentrations of 10⁴, 10⁵, and 10⁶ CFU/ml and injected into the teat canal.
- Data Collection: Clinical symptoms are observed, and mammary tissues are collected for pathological evaluation.
- Advanced Analysis: RNA extraction for qPCR analysis to measure mRNA transcription levels of chemokines, complement factors, and other relevant markers.
Technology readiness level
This model is at Technology Readiness Level 5, indicating that it has been validated in a relevant environment with controlled experimental conditions. Further validation is planned to refine the model and its applications in mastitis research.
About Chinese Academy of Agricultural Sciences
The Chinese Academy of Agricultural Sciences is China’s national comprehensive agricultural research institution headquartered in Beijing, overseeing more than 30 research institutes, a graduate school, and a publishing house. Its network of institutes and experimental stations across diverse agro‑ecological zones supports multi‑site field trials, germplasm evaluation, and large‑scale demonstrations, giving partners practical routes from lab results to regional validation. Industry engagement is organized through a dedicated technology transfer bureau and a center recognized as a National Pilot Center for Technology Transfer, complemented by enterprise partnerships and national technology transfer fairs. Research is supported by national ministries and programs, including the Ministry of Agriculture and Rural Affairs, the National Natural Science Foundation of China, and the Ministry of Science and Technology. Global collaboration is active through formal ties with hundreds of international organizations and companies.
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