Encapsulation of probiotics into dual-crosslinked biopolymer structures
A novel encapsulation strategy enhances probiotic viability using micellar caseins, inulin, trehalose, and dual crosslinking within a gelatin-pectin matrix. This approach improves stability against environmental stressors, making it suitable for shelf-stable probiotic gummy candies.
Overview
This innovative encapsulation technology aims to significantly enhance the viability and functionality of probiotics by employing a multi-layered protection strategy. The method involves encasing probiotics within a micellar casein core, which is subsequently coated with inulin and trehalose. This structure is fortified through dual crosslinking using ionic interactions and covalent bonds, and finally embedded within a gelatin-pectin gel matrix. This comprehensive approach provides a robust barrier against environmental stressors such as heat, moisture, and pH variations, and is particularly suitable for developing shelf-stable probiotic gummy candies.
Technical specifications
Key features:
- Multi-layer encapsulation: Probiotics are first encapsulated in micellar caseins, followed by sequential coatings of inulin and trehalose.
- Dual crosslinking: Utilizes potassium ions and transglutaminase to create ionic and covalent bonds, enhancing the structural integrity of the encapsulation.
- Gel matrix embedding: The encapsulated probiotic cores are embedded in a gelatin-pectin matrix, improving texture and delivery.
- Improved stability: Provides protection against moisture, oxygen, extreme temperatures, and pH levels, maintaining probiotic viability through manufacturing and storage.
Technology readiness level
Currently at Technology Readiness Level 3, this technology has completed initial experimental validations of probiotic core engineering. The next phases involve product integration, optimization, and prototype development for probiotic gummy candies, further assessing viability under extreme conditions.
About Texas A&M University, College Station
Texas A&M University in College Station is a comprehensive public research university and the flagship of The Texas A&M University System, combining broad academic strengths with a strong applied‑research culture. Industry collaborates on the Texas A&M‑RELLIS campus—an integrated education, research and testing environment that supports large‑scale experimentation and proving grounds—and through the Texas A&M Transportation Institute’s facilities in Bryan‑College Station. A statewide extension network connects university expertise to companies and communities across all Texas counties, enabling rapid piloting and deployment. Research is supported by competitive federal funding from agencies such as NSF, NIH, DOE, USDA and DoD, alongside state and industry sponsorship. Texas A&M Innovation provides IP management, licensing and commercialization pathways across the system.
Inquire about this partnering listing
Industry applications
delivered to your inbox.