Enhanced glucoraphanin biosynthesis in broccoli and cabbage through gene editing
This innovative solution enhances glucoraphanin levels in broccoli and cabbage by editing specific genes involved in glucosinolate biosynthesis. By modifying MYB28 and knocking out ADAP and AOP2, this approach aims to increase health-benefiting phytochemicals for commercial cultivation.
Overview
This research focuses on increasing the glucoraphanin content in broccoli and cabbage, which are known for their health benefits due to glucosinolates. By leveraging genetic editing techniques, this solution targets key genes in the glucosinolate biosynthesis pathway to enhance glucoraphanin levels. This approach could lead to commercial varieties of broccoli and cabbage with improved nutritional profiles.
Technical specifications
The solution utilizes targeted gene editing of the MYB28, ADAP, and AOP2 genes. Key features include:
- MYB28 Editing: Introduction of a mutated MYB28 sequence from B. villosa that contains SNPs and a 9-bp deletion, enhancing glucoraphanin biosynthesis.
- ADAP Knockout: Targeting the ADAP gene, a negative regulator of aliphatic glucosinolate biosynthesis, to increase glucoraphanin levels.
- AOP2 Knockout: Editing the functional copy of AOP2 to prevent conversion of glucoraphanin to gluconapin, maintaining higher glucoraphanin levels.
Technology readiness level
The technology is at TRL 3, with individual gene edits of AOP2 and MYB28 already validated to increase glucoraphanin content. Future plans include editing ADAP and combining edits using a protoplast-based method for commercial breeding.
About SEJONG UNIVERSITY
Sejong University is a comprehensive private research university in Seoul, South Korea, with a practical, industry-facing culture. Corporate partners engage through collaborative labs and contract research facilitated by an industry-academic cooperation foundation that streamlines agreements and compliance. A central Seoul location puts talent next to major technology and manufacturing hubs, enabling internships, executive education, and fast site-to-lab collaboration. Research is backed by competitive national programs, including the National Research Foundation of Korea and government ministries, and a dedicated technology transfer office manages IP, licensing, and startup support.
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