Enhancing tomato shelf-life with crispr/cas9 gene editing
This innovative solution utilizes CRISPR/Cas9 technology to enhance the shelf-life and firmness of Moroccan tomatoes by targeting pectin degradation enzymes. The approach promises to reduce post-harvest losses, benefiting farmers and satisfying consumer demands.
Overview
This solution leverages advanced CRISPR/Cas9 gene editing technology to improve the shelf-life and firmness of tomatoes, specifically targeting Moroccan cultivars. By knocking out two key pectin degrading enzymes, polygalacturonase (SlPG2a) and pectate lyase (SlPL), the approach aims to produce firmer tomatoes with extended shelf-life. This innovation is crucial in reducing post-harvest losses, thereby increasing income for farmers and fulfilling consumer expectations for quality produce.
Technical specifications
Key features:
- Utilizes CRISPR/Cas9 for precise genomic editing
- Targets and knocks out SlPG2a and SlPL enzymes responsible for pectin degradation
- Includes in-silico gRNA design, plasmid preparation, and RT-qPCR analysis for gene expression monitoring
- Employs Agrobacterium-mediated genetic transformation for genome editing
- Involves greenhouse and biochemical evaluations to select optimal mutant lines
Technology readiness level
The technology is currently at TRL 3, with ongoing laboratory validation of the CRISPR/Cas9 constructs. Future steps include greenhouse trials, backcrossing to produce DNA-free edited plants, and extensive field testing, which are projected to take approximately three years to complete.
About University Mohammed VI Polytechnic
Mohammed VI Polytechnic University (UM6P) is a Moroccan research university headquartered in Benguerir, focused on applied, industry-facing science and engineering with a pan-African mission. The main campus integrates laboratories with testbeds, maker spaces, and demonstration sites, enabling companies to prototype and validate solutions in operational environments. Close alignment with OCP Group provides access to industrial facilities, data, and talent pipelines, while an on-campus innovation district supports co-location of startups and corporate R&D. Research is supported by Moroccan national programs and competitive international funding. A dedicated technology transfer office and venture mechanisms streamline IP protection, licensing, and new venture creation.
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