Gene editing of tomato for enhanced shelf-life and nutrition

Overview

This innovative solution leverages CRISPR/Cas9 gene editing technology to target specific genes in tomatoes, aiming to extend their shelf-life and enhance nutritional content. By editing the SlFSR and SlLOB1 genes, the project aims to reduce post-harvest losses and retain essential nutrients, which is crucial for regions with limited dietary diversity. This approach can lead to the development of elite hybrid tomato varieties with improved storage and health benefits, addressing both agricultural and nutritional challenges.

Technical specifications

• Gene Targets: The solution focuses on the targeted editing of SlFSR and SlLOB1 genes, known to regulate cell wall-related genes and fruit ripening.
• Methodology: Utilizes in-silico design of guide RNA and primers, followed by CRISPR/Cas9 constructs development for precise genetic modifications.
• Cultivars: Four farmer-preferred tomato cultivars have been selected for this research.
• Procedures: Advanced in-vitro regeneration and Agrobacterium-mediated regeneration protocols are adapted for the cultivars under study.
• Validation: Includes sequencing, gRNA design, screening for point mutations, and assessing shelf-life and quality parameters.

Technology readiness level

The project is currently at Technology Readiness Level 3, indicating that active research and development are underway. The solution is being validated in controlled environments with ongoing support from international and local partners. Future steps include further development of CRISPR constructs, field testing, and regulatory compliance assessments. The project seeks co-development partnerships to advance from lab proof-of-concept to final product development.