Field rapid detection of DNA/RNA sequences by magnetic hybridization technique

Overview

This solution addresses the critical need for rapid, on-site detection of specific DNA and RNA sequences in leaf and seed material. By combining a patented magnetic in-vitro diagnostic (IVD) device with an established non-radioactive molecular hybridization technique, the platform enables experts to identify plant pathogens directly in the field, eliminating the delays associated with routine laboratory analysis. Faster detection supports timely interventions, helping reduce crop losses and improve agricultural outcomes.

The technology brings together two complementary innovations: a cartridge-based magnetic readout platform developed by BioEclosion, and a hybridization probe methodology developed over more than 30 years by the Virology group at the Instituto de Biología Molecular y Celular de Plantas (IBMCP). The integration aims to deliver a versatile diagnostic tool applicable across plant health, food safety, and environmental monitoring.

Technical specifications

Key features:

• Patented magnetic IVD cartridge that simplifies test performance and delivers rapid, easy-to-interpret, quantitative results in non-laboratory settings
• Non-radioactive molecular hybridization probes validated through numerous publications and two patents, capable of targeting specific DNA or RNA sequences associated with plant viruses and viroids
• Magnetic particle-based readout that removes the need for radioactive labels while maintaining sensitivity and specificity
• Portable, field-ready design suitable for use by non-specialist operators outside conventional laboratory infrastructure
• Broad application potential spanning plant pathogen detection, food safety screening, and environmental monitoring

How it works: The platform uses synthesized hybridization probes designed to bind specific genetic targets in plant tissue samples. The magnetic IVD device detects and quantifies the bound targets through its patented magnetic readout, producing results that can be interpreted at the point of use without radioisotopes or complex laboratory equipment.

Technology readiness level

The magnetic IVD device is currently undergoing analytical validation studies in collaboration with academic partners, with trial studies planned for Q3 2024. The core magnetic technology is supported by 25 years of scientific research led by the device's lead scientist. The hybridization probe methodology has already reached commercial maturity, with its laboratory version implemented by various companies and laboratories for detecting plant viruses and viroids.

The proposed project outlines a 1.5-year development pathway with three work packages: probe design and laboratory validation, integration of probes with the magnetic device and proof-of-concept optimization, and final validation of a minimum viable product (MVP) against gold-standard laboratory methods. The partnership is seeking funding for key personnel during the experimental phase, as well as expertise to define target needs and provide test samples for proof-of-concept and MVP validation.