Free radical scavenging technology for hair loss prevention

Overview

This research presents a novel approach to combating hair loss through the measurement of free radical scavenging activities of natural botanicals. Free radicals, such as reactive oxygen species and nitrogen species, are implicated in the pathogenesis of hair loss. By selecting plants that suppress oxidative stress in hair follicle cells, this method aims to mitigate hair loss effects. This technology leverages fluorescence and chemiluminescence assays to evaluate the antioxidant power of various botanicals, offering a scientific basis for identifying potent natural ingredients.

Technical specifications

The core technology involves generating specific free radicals, such as hydroxyl radicals and hydrogen peroxide, under physiological conditions in vitro. The antioxidant activity of botanicals is assessed by the reduction in fluorescence or chemiluminescence, indicating their efficacy in neutralizing oxidative stress. Advanced assays, including the oxidation of DCHF by Fenton reagent and peroxynitrite, are utilized to select samples with superior antioxidant properties. Subsequent evaluations of hair growth efficacy are conducted in vitro, ex vivo, and in vivo, using isolated vibrissa follicles, cultured mouse skin, and intradermal injections in mice, respectively.

Technology readiness level

The current technology readiness level is 3, indicating that this method has been validated in laboratory settings and is in the initial stages of experimental proof of concept. Further validation and development are planned to advance its readiness for commercial applications.