Functional analysis of CYC-B promoter SNPs for enhanced β-carotene in tomatoes

Overview

This research focuses on the functional analysis of promoter SNPs in the CYC-B gene to enhance β-carotene accumulation in tomatoes, a crucial nutrient for human health. By analyzing 132 EMS-mutagenized tomato lines, the project aims to identify promoter variations that significantly increase β-carotene levels, offering a pathway to biofortified tomato varieties with improved nutritional profiles.

Technical specifications

• Core technology: Identification of SNPs in the CYC-B promoter region, using whole-genome sequencing of EMS-mutagenized tomato lines.
• Validation methods: Dual-luciferase reporter assays to measure promoter activity and functional impact of SNPs.
• Experimental approach: Amplification and cloning of promoter variants, transient expression in Nicotiana benthamiana leaves, and luciferase activity measurement.
• Analysis: Correlation of SNP presence with CYC-B expression levels and β-carotene content.

Technology readiness level

The research is at TRL 3, with ongoing experimental validation to assess the functional impact of identified SNPs. This stage involves in-silico analysis and dual-luciferase reporter assays to evaluate the regulatory potential of promoter variants.