Gene-edited biofortified tomato with enhanced beta-carotene
This project focuses on developing a biofortified tomato with significantly increased beta-carotene levels through gene editing. By enhancing the lycopene-beta-cyclase (LCYB) enzyme efficiency, the nutritional value of tomatoes is improved, addressing vitamin A deficiency.
Overview
This innovative research aims to develop a biofortified tomato variety with increased beta-carotene levels to combat global vitamin A deficiency. The approach involves gene-editing the lycopene-beta-cyclase (LCYB) enzyme, which plays a crucial role in converting lycopene to beta-carotene. By enhancing LCYB's efficiency, the project's goal is to significantly boost beta-carotene content in tomatoes, thereby enhancing their nutritional value and providing a dietary solution to vitamin A deficiency.
Technical specifications
Key features:
- Gene-editing of the LCYB enzyme to enhance beta-carotene synthesis.
- Utilization of a transgenic construct with 'LCYB-like activity' tested in experimental settings.
- Future plans involve identifying target residues for modification and using in vitro mutagenesis.
- Implementation of sgRNA cassettes via Golden Gate and BioBrick assembly methods.
- Evaluation through recombinant bacterial systems to optimize carotenoid profiles before plant application.
Technology readiness level
The current development stage of this biofortified tomato is at Technology Readiness Level 2, indicating that the concept has been formulated and initial experimental research has been conducted. Further validation and refinement of the gene-editing strategy are planned to progress toward higher readiness levels.
About University of Florida
The University of Florida is a comprehensive public research university with a broad academic and research portfolio and a statewide presence. Industry collaborates through co-located labs and shared core facilities and through an integrated academic health system that accelerates clinical translation. A statewide extension network and multiple research and education sites connect companies with field-scale testing and rapid deployment, while incubators and an adjacent innovation district provide pathways from lab to market. Research is supported by competitive funding from major federal agencies such as NIH, NSF, USDA, and DOE. A dedicated technology transfer office supports IP, licensing, and startup formation.
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