Genetic variation analysis for protein extraction efficiency in pulse crops

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Research collaboration opportunity to identify genetic sources of variation in seed structure that improve protein extraction and fractionation efficiency in pulse crops. The project leverages multispectral and hyperspectral imaging on assembled genetic panels of Phaseolus and Cicer species to discover genotypes with superior protein extraction characteristics, supporting breeding for improved industrial processing outcomes.

Overview

This research initiative seeks to identify genetic sources of variation in seed structure and cellular organization that lead to higher efficiency of protein extraction and fractionation in pulse crops. By examining genetic panels of Phaseolus spp. (common beans) and Cicer spp. (chickpeas), the project aims to discover superior genotypes that can enhance industrial protein extraction processes. The approach combines high-throughput phenotyping with genetic analysis to address a key bottleneck in breeding for protein quality and extraction efficiency. Deploying desirable traits at the primary production stage through breeding offers a sustainable and safe pathway to reduce industrial processing costs and improve efficiency.

Technical specifications
  • Assembled genetic variation panels of approximately 500 entries each for Phaseolus spp. and Cicer spp. sourced from diverse genepools
  • Evaluation of panels for total protein content and field production suitability in Northeastern North America
  • Multispectral and hyperspectral imaging capabilities for high-throughput characterization of seed and protein traits
  • Integration of plant physiology, biochemistry, food science, and microscopy expertise
  • Planned genome-wide association studies to link genetic markers with protein extraction efficiency traits
  • Capacity to characterize contrasting protein levels and protein extraction characteristics across diverse genotypes
Technology readiness level

The research is currently at an early-to-mid stage of development. The team has successfully assembled genetic panels and completed initial evaluations for total protein and field production suitability. The next phase requires a data scientist collaborator to analyze multispectral and hyperspectral imaging data, characterize genotypes for protein extraction traits, and conduct genome-wide association studies. This collaboration would accelerate the identification of genetic markers linked to improved protein extraction efficiency and advance breeding strategies for these important pulse crop quality characteristics.


About Pulse Breeding, Genomics and Phenomics Research Laboratory

The Pulse Breeding and Genetics Lab, located within the Department of Plant Science at McGill University, is a research group focused on the genetic improvement of pulse legumes, including common beans, chickpeas, and dry peas. The lab utilizes field data to conduct research in areas such as genomics, quantitative genetics, abiotic stress tolerance, disease resistance, and high-throughput phenotyping. By integrating emerging technologies into agricultural systems, the team aims to develop superior cultivars that enhance yield, quality, and nutritional profiles for sustainable production. Their work combines fundamental research with applied plant breeding to generate new knowledge and drive agricultural innovation.

This research is critical for supporting the pulse industry, farmers, and end-users by providing solutions that improve productivity and sustainability in Quebec, Canada, and internationally. The lab engages in collaborative efforts with the private and public sectors, offering opportunities for industrial partners to leverage their expertise and equipment for accelerated product development and R&D. Furthermore, the laboratory plays a significant role in education by training MS and PhD students in the fields of plant breeding and genetics, ensuring the development of scientific leadership to advance global food security and nutrition.

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