Genome editing for extended shelf-life in broccoli
This innovative approach leverages genome editing to extend broccoli's shelf-life by targeting specific STAY-GREEN (SGR) genes responsible for post-harvest senescence, ultimately reducing chlorophyll degradation and delaying yellowing.
Overview
Our research focuses on extending the shelf-life of broccoli through genome editing techniques. By targeting specific STAY-GREEN (SGR) genes, which play a critical role in chlorophyll degradation during post-harvest senescence, we aim to significantly delay the yellowing process. This innovative approach not only enhances the visual appeal and nutritional quality of broccoli but also reduces food waste by prolonging freshness.
Technical specifications
Key features:
- Identification of six BoSGR family members in broccoli, with three (BoSGR1, BoSGR3-1, BoSGR7) significantly upregulated during post-harvest senescence
- Use of sgRNAs for precise genome editing of specific BoSGR genes
- Application of a protoplast-based RNP method to achieve efficient genome editing
- Analysis of chlorophyll-degrading gene expression to assess the impact on senescence
Technology readiness level
Our research is currently at Technology Readiness Level 3, focusing on experimental proof of concept. Future validation includes designing sgRNAs and testing their efficiency, followed by editing application and analysis of resulting broccoli lines to measure improvements in shelf-life.
About SEJONG UNIVERSITY
Sejong University is a comprehensive private research university in Seoul, South Korea, with a practical, industry-facing culture. Corporate partners engage through collaborative labs and contract research facilitated by an industry-academic cooperation foundation that streamlines agreements and compliance. A central Seoul location puts talent next to major technology and manufacturing hubs, enabling internships, executive education, and fast site-to-lab collaboration. Research is backed by competitive national programs, including the National Research Foundation of Korea and government ministries, and a dedicated technology transfer office manages IP, licensing, and startup support.
Inquire about this partnering listing
Industry applications
delivered to your inbox.