High-throughput label-free CARS imaging for wound healing screening

Overview

The proposed solution is a high-throughput, label-free optical imaging platform that leverages coherent anti-Stokes Raman scattering microscopy (CARS) and second harmonic generation (SHG). This innovative system is designed to enhance the screening of wound healing processes in both 2D and 3D skin and epithelial tissue models. By enabling the assessment of key regenerative processes such as extracellular matrix (ECM) production, cell migration, proliferation, and lipid/protein distribution, this platform offers a non-invasive and chemically specific approach that provides higher biological relevance and throughput than traditional assays.

Technical specifications

Key features:

• CARS and SHG Integration: Combines CARS microscopy with SHG for detailed imaging without the need for fluorescent labels.
• High Throughput: Compatible with standard 96-well plates, allowing scalable and automated compound screening.
• Quantitative Readouts: Provides real-time analysis of ECM remodeling, cell migration, and multicellular interactions.
• Multiplexed Datasets: Delivers rich data from live or fixed samples, capturing dynamic regenerative behaviors.
• Automated Workflows: Supports automated compound dosing, live-cell kinetic imaging, and wound-closure assays.

Technology readiness level

The current technology readiness level (TRL) is 4, indicating that the technology has been validated in a lab setting. Future plans include a two-phase sponsored research project to further optimize and validate the platform for broader applications, ensuring robust performance and data accuracy.