Hybridization impact on maize seed and seedling microbiomes

Overview

This research focuses on the impact of hybridization on the assembly of maize seed and seedling microbiomes. By understanding how hybridization alters microbial communities, this study aims to optimize seed microbiomes to enhance crop resilience and performance. The project examines whether the microbiomes of hybrid seeds differ from their parental lines and how these communities influence seedling development and agronomic traits.

Technical specifications

The research utilizes advanced sequencing techniques, including 16S rRNA and ITS for bacteria and fungi, respectively, and metagenome sequencing to analyze the microbial diversity, taxonomic composition, and functional profiles. Key methodologies involve:

• Assessing the repeatability of hybridization effects using three independent batches of hybrids
• Inoculating seeds with soil slurries from maize monoculture and forest soil
• Sequencing 500 amplicon samples with Illumina NextSeq and further metagenome sequencing on a PromethION
• Data analysis using R and Python for simulations and modeling

Technology readiness level

The project is currently at Technology Readiness Level 4, indicating a fundamental understanding of the microbial interactions in controlled environments, with ongoing validation efforts to assess repeatability and broader application potential.