Impact of fungicides on wheat leaf cuticle structure and composition
Research exploring how fungicides alter wheat leaf cuticle composition and structure to enhance sustainable plant protection strategies. Methods include microscopy, staining, and mass spectrometry to assess changes in cuticle porosity and permeability.
Overview
This research investigates the effects of fungicides on the composition, structure, and permeability of wheat leaf cuticles. These cuticles are the first line of contact for fungicides, and understanding their alterations can enhance sustainable plant protection. The study aims to reveal structural and chemical changes in cuticles, providing insights into more effective and safe agricultural practices.
Technical specifications
- Methods Used: The research involves applying two fungicides at varying concentrations on wheat leaves. Techniques such as lipid isolation, staining, scanning electron microscopy (SEM), and fluorescence microscopy are used to assess changes in cuticle volume, porosity, and integrity.
- Analysis Tools: Gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS), and mass spectrometry imaging (MSI) are employed to study chemical alterations. Advanced methods like Raman spectroscopy are explored for detailed pesticide distribution analysis.
- Focus Areas: The study examines cuticle porosity, fractures, and cuticle-cell wall interactions, specifically focusing on cellulose and pectin distributions.
Technology readiness level
This research is currently at Technology Readiness Level 2, involving the formulation of technology concepts and applications.
About Sesallab R&D and Consultancy Company
SESAL LAB is an R&D and consultancy company founded in 2020 by Prof. Dr. Nüzhet Cenk Sesal to bridge academic knowledge with industrial applications. The company operates by leveraging a scientific, multidisciplinary approach to solve industrial problems, offering services in research, development, and project consultancy. Its laboratory infrastructure supports activities ranging from microbiology and molecular biology to product design and testing, enabling the transition of prototypes into industrial-scale production. The team specializes in identifying problem sources through scientific analysis, utilizing expertise in engineering, pharmacy, chemistry, and biology to develop innovative, customized solutions for diverse clients.
By facilitating partnerships between universities and the industry, SESAL LAB aims to enhance national and international competitiveness and reduce foreign dependency. The company has a history of conducting numerous national and international projects, including collaborations with institutions in Japan, Italy, Korea, and Spain, and has contributed to the establishment of several technology-based firms. Through its R&D support, the company assists partners in areas such as patent development, business excellence, and the realization of innovative ideas, serving as a solution partner for sectors including construction, textiles, medicine, and agriculture.
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