Instrument-free semi-quantitative lateral flow tests for micronutrients
This innovative lateral flow test enables instrument-free, semi-quantitative detection of micronutrients using a gradient-based assay. It replaces traditional intensity measurements with line counting, offering a simple and effective solution for point-of-care testing.
Overview
The proposed technology introduces a novel approach to lateral flow testing by utilizing a gradient-based assay for semi-quantitative detection of micronutrients. Unlike conventional methods that rely on intensity measurements requiring smartphones or dedicated readers, this solution quantifies results by counting developed lines. This approach is particularly suitable for competitive immunoassays, where the number of lines is inversely proportional to the analyte concentration. The technology is adaptable to various micronutrient detections and offers significant advantages for instrument-free, point-of-care diagnostics.
Technical specifications
- Gradient-based assay: Utilizes multiple test lines with increasing concentrations of capture agents to enhance quantitative resolution.
- Competitive immunoassay format: Ideal for small molecule detection, with line development indicating analyte concentration.
- Current application: Successfully demonstrated for SARS-CoV-2 neutralizing antibodies.
- Future adaptation: Plans to target 25-hydroxyvitamin D3 using gold-conjugated antibodies for detection.
- Whole blood compatibility: Development includes integration of blood separation filters to reduce background noise in whole blood samples.
Technology readiness level
This technology is currently at TRL 2, having demonstrated the initial embodiment for antibody detection. Future work involves adapting and optimizing the test for 25-hydroxyvitamin D3 detection and improving its application with whole blood samples.
About Toronto Metropolitan University
Toronto Metropolitan University is a comprehensive public research university in the heart of downtown Toronto, recognized for experiential, career‑integrated education and a diverse, industry‑connected student body. Industry access is embedded through co‑located and community‑based platforms, including the DMZ startup incubator and a university‑wide Zone Learning network that connects companies with faculty and student innovators. A dedicated Centre for Urban Innovation hosts collaborative labs and pilot spaces, while the Brampton‑based School of Medicine—affiliated with William Osler Health System—creates direct pathways for clinical training and partnership; the Rogers Cybersecure Catalyst extends capacity in workforce development and applied solutions. Research is supported by competitive federal and provincial funding, including NSERC, CIHR, SSHRC and the Canada Foundation for Innovation. A formal commercialization framework and IP policy provide structured support for protection, licensing and venture creation.
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