Lamp-on-a-strip rapid genetic feature detection platform
Field-deployable lateral flow platform combining loop-mediated isothermal amplification (LAMP) with quantum dot nanotechnology for SNP and genetic feature detection. Designed for portable, point-of-use testing with sensitivity comparable to RT-qPCR and minimal sample processing.
Overview
This solution integrates loop-mediated isothermal amplification (LAMP) with a lateral flow strip format to enable rapid, field-based detection of single nucleotide polymorphisms (SNPs) and other genetic features. The platform is designed for portability, ease of use, and sensitivity comparable to laboratory-based RT-qPCR, while requiring only minimal sample processing. Potential applications include GMO detection, infectious disease diagnostics, and other use cases requiring portable molecular testing outside of laboratory settings.
Technical specifications
- LAMP amplification combined with lateral flow readout enables isothermal DNA amplification without the need for thermal cycling equipment
- Endpoint visualisation on the strip as fluorescent or coloured lines, leveraging proprietary quantum dot nanoparticle technology for enhanced signal clarity
- Quantum dot nanoparticles engineered for stability in assay buffers and capable of conjugation with biologically active molecules
- Minimal sample processing from swab-style inputs, reducing the need for laboratory infrastructure
- Proprietary laboratory equipment including automated stripers for protein application on nitrocellulose and electronic readers for fluorescence and colorimetric quantification
- Sensitivity demonstrated in chlamydia detection to be comparable with RT-qPCR
Technology readiness level
The core lateral flow-LAMP platform has been validated for the detection of chlamydial DNA. The extension of LAMP chemistry to specifically detect SNPs and additional genetic features has not yet been validated experimentally. Future development steps include identifying specific genetic targets of interest, designing LAMP primers, and testing primers in standard LAMP assays followed by evaluation within the lateral flow-LAMP system.
About Radetec Diagnostics
Radetec Diagnostics is a Melbourne-based bio-nanotechnology company that specializes in the production and application of highly fluorescent quantum dot nanoparticles. These nanoparticles are engineered to be stable in buffers and can be conjugated with various biologically active molecules, such as antibodies. Beyond nanoparticle synthesis, the company develops specialized laboratory equipment for lateral flow assay development, including automated stripers for protein application on nitrocellulose and electronic readers for fluorescence and colorimetric quantification. By combining this proprietary nanotechnology with molecular and serological assays, Radetec creates highly sensitive, cost-effective point-of-care diagnostic tools.
These innovations are primarily directed toward revolutionizing the detection of infectious diseases, including sexually transmitted infections and animal health conditions. The company’s diagnostic platform aims to improve accessibility to rapid, private testing, helping to mitigate the societal stigma and high costs associated with traditional lab-based molecular testing. Through research partnerships and custom synthesis services, Radetec also supports the broader scientific community in developing tailored nanomaterials and lateral flow applications for various biomedical and industrial research needs.
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