Multiplex detection of Salmonella in chocolate using Luminex assay
A nucleotide-based Luminex assay for detecting multiple Salmonella strains in chocolate, leveraging genomic data for enhanced specificity and sensitivity. The assay is in development for robust real-world application, currently at TRL 4.
Overview
This innovative solution utilizes a nucleotide-based multiplex detection platform, the Luminex assay, to accurately identify multiple strains of Salmonella in chocolate products. By leveraging a vast genomic dataset of nearly 1000 Salmonella genomes, the technology aims to enhance detection accuracy and specificity through optimized microbial enrichment techniques. The platform is designed to provide sensitive, specific, and rapid detection, addressing the critical need for food safety in chocolate production.
Technical specifications
- Nucleotide-based multiplex detection: Utilizes the Luminex platform for simultaneous detection of multiple Salmonella strains.
- Genomic data-driven design: Employs a unique dataset of Salmonella genomes to design specific target regions for effective primer selection.
- Optimized enrichment protocols: Enhances assay sensitivity and specificity through in-house developed microbial enrichment techniques.
- Validation steps: Includes bioinformatic analysis, primer validation on pure DNA, and real-world testing with chocolate samples.
Technology readiness level
The technology is currently at TRL 4, indicating that it has been validated at a laboratory level. Future validation involves further testing under real-world conditions, including the application to chocolate samples with varied contamination levels, to ensure robustness and effectiveness in practical settings.
About Wageningen University and Research
Wageningen University & Research is a life sciences–focused public university combined with mission-driven research institutes, integrating fundamental, applied, and field-based R&D at scale. On Wageningen Campus, companies co-locate with WUR teams and use shared pilot plants, advanced analytical labs, and controlled‑environment facilities for rapid prototyping. Being in the Food Valley cluster provides tight links to global corporates, SMEs, and startups through living labs and a strong regional talent pipeline. Research is backed by competitive funding from the Dutch Research Council, European Union programs, and national ministries, alongside significant contract research for industry. A dedicated technology transfer office and campus incubators streamline IP, licensing, and spin‑out formation for corporate partners and entrepreneurs.
Inquire about this partnering listing
Industry applications
delivered to your inbox.