Rapid and low-cost salmonella detection in chocolate using RT-PCR and multiplex D3 array

Overview

The innovative Serox Assay by PathogenDx offers a breakthrough in food safety testing by enabling rapid and cost-effective detection of Salmonella in chocolate. Utilizing a combination of rapid enrichment protocols and advanced RT-PCR multiplexing, this assay can identify Salmonella down to 1 CFU per 325 grams. It further differentiates up to 13 atypical Salmonella subspecies in a single test, making it a powerful tool for ensuring the safety of chocolate products.

Technical specifications

Key features:

• Rapid enrichment: Overcomes statistical limitations in challenging matrices like chocolate with emulsifiers to eliminate PCR inhibitors.
• Multiplex detection: The D3 Array allows for simultaneous detection and differentiation at the DNA or RNA level.
• High sensitivity: Utilizes RNA's higher copy number for superior amplification, achieving detection limits of <0.01 cell equivalence per reaction.
• Enhanced binding surface: The three-dimensional D3 Array design increases binding surface area by 100x, enhancing single-gene sensitivity.
• N=6 probe replication: Distinguishes 1 CFU targets from background noise with SNP level resolution.

Technology readiness level

This technology is at TRL 6, indicating it has been demonstrated in a relevant environment. Future validation steps include optimizing sample preparation, morphing the DNA-based assay to an RNA-based assay, enhancing the limit of detection, and validating with a panel of 10 Salmonella serovars in chocolate suspensions.