Multiplex LNA probe point-of-care DNA/RNA detection system
A portable detection toolkit combining rapid dip-stick nucleic acid extraction with multiplex Locked Nucleic Acid (LNA) fluorescence probes and an automated fluorescence reader. Designed for field-based DNA/RNA detection in under 30 minutes without trained personnel, with applications in agriculture, biosecurity, and clinical diagnostics.
Overview
This solution offers a complete point-of-care detection system for DNA and RNA targets, integrating rapid dip-stick nucleic acid extraction with multiplex Locked Nucleic Acid (LNA) fluorescence probes and an automated fluorescence detection reader. The system addresses a key limitation of conventional laboratory-based qPCR workflows by eliminating the need to transport samples to centralized labs, enabling on-site analysis in under 30 minutes. By combining lyophilized reaction tubes, machine-learning-assisted result interpretation, and a user-friendly interface, the toolkit is designed for use by non-specialist personnel in field, greenhouse, warehouse, or clinical environments.
Technical specifications
Key features:
- Dip-stick extraction: A field-validated nucleic acid extraction method that processes samples in under 30 seconds with high reproducibility and no requirement for trained personnel
- Multiplex LNA probes: Capable of detecting both long DNA fragments and single nucleotide polymorphisms (SNPs) in a single reaction, with up to 32 samples analyzed per run
- Rapid analysis time: Optimized multiplex probe workflow delivers results in approximately 30 minutes
- Automated fluorescence detection: Integrated reader system for objective, consistent signal interpretation
- Lyophilized reaction tubes: Shelf-stable, ready-to-use format simplifies deployment in resource-limited settings
- AI and machine learning interface: User-friendly software layer that streamlines result analysis and reduces operator burden
- Flexible target design: Compatible with custom probe and primer design for specific genes, SNPs, or long insertions
Technology readiness level
The core technologies have been individually validated. The dip-stick extraction method has been demonstrated under laboratory, greenhouse, and field conditions, with proven reproducibility and sub-two-minute processing times. Multiplex LNA probe protocols have been optimized to handle 32 samples per run. Current and planned validation activities include optimization of extraction protocols for target crops, design and evaluation of multiplex probes for SNPs and long insertions, development of the automated reader and user interface, and progressive evaluation through in vitro, greenhouse, and field/warehouse assays. The system is at an advanced integration stage, moving from validated individual components toward a fully assembled, field-ready toolkit.
About Instituto Tecnológico de Costa Rica
Instituto Tecnológico de Costa Rica (TEC) is a public, STEM‑oriented university with a multi‑campus presence across Cartago, San José, Alajuela, and San Carlos, combining applied research with technology extension. Through its Centro de Vinculación, TEC connects companies and public agencies with faculty and research units for contract problem‑solving, customized training, and long‑term partnerships. Technology transfer and continuing education centers embedded on the campuses provide access to specialized laboratories and training, enabling rapid engagement with industry partners; international collaboration infrastructure includes K‑Lab in San Carlos developed with South Korea’s NIPA. Research is supported by national science and innovation agencies and by competitive international programs, including EU Erasmus+ collaborations. A dedicated technology transfer office advances IP management, licensing, and entrepreneurship.
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