Plant Extract-Based Nematicidal Solution for Sustainable Potato Crop Protection

Overview

This research proposes a sustainable, biology-based approach to controlling the potato cyst nematode Globodera rostochiensis, a major pest responsible for significant yield losses in potato cultivation. The solution involves the application of plant extracts from marigolds (Tagetes) and calendula (Calendula officinalis) as natural nematicidal agents. By introducing these botanical extracts into nematode-infested soil, the project aims to suppress larval development and reduce cyst formation, offering potato growers an environmentally responsible alternative to conventional chemical nematicides.

The value proposition centers on reducing dependence on synthetic agrochemicals, minimizing soil and water contamination, and supporting integrated pest management practices. The research is conducted by the Institute of Plant Protection of the National Academy of Agrarian Sciences of Ukraine, bringing established expertise in plant nematology and soil pest management.

Technical specifications

Research approach:

• Target organism: Globodera rostochiensis, the golden potato cyst nematode, evaluated through in vitro laboratory assays
• Active agents: Botanical extracts from marigold (Tagetes) and calendula (Calendula officinalis) plants
• Application protocol: Extracts introduced into nematode-infested soil at three concentration rates, applied twice during the potato growing season
• Experimental design: Potato host plants grown in soil inoculated with 1,500 larvae and eggs per 100 cm³ of soil; eight variants tested in four replicates (n = 32)
• Assessment metrics: Percentage reduction in nematode larvae and eggs compared to control; larval movement activity as an indicator of nematicidal efficacy
• Validation pipeline: Dose-response screening to identify the three most effective concentrations, followed by field-scale evaluation of cyst reduction at the end of the potato growing season

Key features:

• Environmentally benign nematicidal action derived from natural plant compounds
• Compatibility with organic and integrated pest management systems
• Targeted suppression of nematode development and reproduction cycles

Technology readiness level

This research is at an early-to-mid experimental stage (TRL 3–4). Laboratory-based in vitro evaluation of nematicidal activity is established, and a structured validation protocol using pot and field-scale trials with potatoes as the host plant is defined. The project timeline is approximately one year from initiation through completion of validation. The research team is seeking partners who can provide equipment for behavioral testing of nematode larvae and funding to support key personnel. Successful validation would position the product for further field trials and potential commercial development as a bio-based nematicide for the potato production sector.