Precision rnai biopesticide for targeted western corn rootworm control

Overview

Our innovative biopesticide leverages genetically engineered inactivated yeast to express double-stranded RNA (dsRNA) that targets essential genes in Western Corn Rootworm (WCR). Once ingested by WCR, this dsRNA triggers RNA interference (RNAi), effectively silencing crucial genes and causing mortality. This solution is specifically designed to be species-specific, environmentally safe, and seamlessly integrates with existing integrated pest management (IPM) systems. It represents a sustainable shift from traditional chemical insecticides, addressing the growing resistance issues.

Technical specifications

• Genetic Engineering: Utilizes inactivated baker’s yeast (Saccharomyces cerevisiae) engineered to express dsRNA.
• Species-Specific Targeting: Designed to target essential genes in Western Corn Rootworm through RNAi.
• Environmental Stability: Protects dsRNA from degradation, ensuring effective oral delivery via soil or seed applications.
• Validation: Proven efficacy in Colorado potato beetle field trials and WCR laboratory assays.
• Application Methods: Compatible with soil, seed, and bait delivery formats, modeled in soil microcosms.
• Fermentation and Formulation: Optimized for industrial fermentation, maintaining insecticidal efficacy.

Technology readiness level

Currently at Technology Readiness Level 4, this biopesticide has been validated in laboratory settings with targeted gene and strain selection. The next phase involves optimizing the platform for scalable application and conducting field trials to transition from lab-validated strains to field-ready prototypes.