Prime editing of uorfs for gene expression modulation in rice
A novel platform using Prime Editing to engineer upstream Open Reading Frames (uORFs) in rice, enabling precise modulation of gene expression to improve agronomic traits without altering native genetic sequences.
Overview
This innovative solution employs Prime Editing to modulate gene expression in rice by engineering upstream Open Reading Frames (uORFs) in the 5' UTR of target genes. By inserting or abolishing uORFs, this platform acts as a "translational rheostat," allowing for fine-tuning of protein levels by 30-80% without changing the native promoter or coding sequence. This method offers a sophisticated, post-transcriptional control mechanism for enhancing agronomically important traits in rice, providing a powerful tool for crop improvement that mimics natural genetic variation.
Technical specifications
- Prime Editing technology: Utilizes precise insertions and modifications in the 5' UTR to modulate gene expression.
- Dual-Control Approach: Inserts new uORFs for down-regulation and removes start codons of existing uORFs for up-regulation.
- Non-GMO strategy: Final edited plants are transgene-free, making subtle modifications that align with natural alleles.
- Validation Phases:
- Phase 1: High-throughput screening to optimize uORF modifications using a dual-luciferase reporter system.
- Phase 2: Stable transformation and analysis in rice to confirm uORF functionality and agronomic impact.
Technology readiness level
The current stage of development is at Technology Readiness Level 3, involving proof-of-concept validation in controlled environments.
About Vietnam Academy of Science and Technology
Vietnam Academy of Science and Technology is Vietnam’s national academy and a multi‑institute research organization headquartered in Hanoi, pairing basic and applied research with a graduate university that trains master’s and doctoral talent. A nationwide network of institutes, specialized labs, field stations, and shared facilities enables co‑located collaboration with regional industries and government via contract research and analytical testing. Locations near industrial corridors in Hanoi, Ho Chi Minh City, and key provinces support joint problem‑solving, pilot‑scale prototyping, and technology validation close to manufacturing supply chains. Research is primarily funded by Vietnam’s national government, with additional competitive support from international partners; a central technology transfer office manages IP, licensing, and industry‑sponsored research and spinout activity.
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